Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 134, Issue 9, Pages 4124-4131Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja2078812
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Funding
- Natural Sciences and Engineering Research Council of Canada
- Canada Foundation for Innovation
- Canada Research Chairs
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It has long been recognized that hydrogen bonds formed by protein backbone amides with cysteinyl S-gamma atoms play important roles in modulating the functional and structural properties of the iron-sulfur centers in proteins. Here we use single molecule atomic force microscopy, cyclic voltammetry, and protein engineering techniques to investigate directly how the strength of N-H center dot center dot center dot S-gamma hydrogen bonds in the secondary coordination sphere affects the mechanical stability of Fe(III)-thiolate bonds of rubredoxin. Our results show that the mechanical stability of Fe(III)-thiolate bonds in rubredoxin correlates with the strength of N-H center dot center dot center dot S-gamma hydrogen bonds as reflected by the midpoint reduction potential, providing direct evidence that N-H center dot center dot center dot S-gamma hydrogen bonds play important roles in modulating the mechanical and kinetic properties of the Fe(III)-thiolate bonds of iron-sulfur proteins and corroborating the important roles of the protein environment in tuning the properties of metal-thiolate bonds.
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