Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 134, Issue 46, Pages 18908-18911Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja3082439
Keywords
-
Categories
Funding
- National Basic Research Program of China [2010CB732402]
- National Science Foundation of China [21075104]
- National Instrumentation Program [2011YQ03012412]
- Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]
- National Fund for Fostering Talents of Basic Science [J1030415]
Ask authors/readers for more resources
Noninvasive and accurate measurement of intracellular temperature is of great significance in biology and medicine. This paper describes a safe, stable, and accurate intracellular nano-thermometer based on an L-DNA molecular beacon (L-MB), a dual-labeled hairpin oligonucleotide built from the optical isomer of naturally occurring D-DNA. Relying on the temperature responsive hairpin structure and the FRET signaling mechanism of MBs, the fluorescence of L-MBs is quenched below the melting temperature and enhanced with increasing temperature. Because of the excellent reversibility and tunable response range, L-MBs are very suitable for temperature sensing. More importantly, the non-natural L-DNA backbone prevents the L-MBs from binding to cellular nucleic acids and proteins as well as from being digested by nucleases inside the cells, thus ensuring excellent stability and accuracy of the nano-thermometer in a complex cellular environment. The L-MB nano-thermometer was used for the photothermal study of Pd nanosheets in living cells, establishing the nano-thermometer as a useful tool for intracellular temperature measurement.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available