Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 134, Issue 13, Pages 5807-5816Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja208993r
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Funding
- Singapore Ministry of Education [RG62/07, RG72/10]
- Yousef Jameel scholarship
- Association pour la recherche sur le cancer
- Region Aquitaine
- Fondation pour la Recherche Medicale (F.R.M.)
- INCa
- ANR [F-DNA, G4-TOOLBOX]
- Ligue Nationale contre le Cancer (LNCC) [EL2007.LNCC/AN, EL2010.LNCC/AN]
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CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nucleotide (nt) tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 to 20 kb, maintained by mendelian inheritance but also subject to germline instability. To provide insights on the biological role of CEB25, we interrogated its structural features. We report the NMR structure of the G-quadruplex formed by the conserved 26-nt G-rich fragment of the CEB25 motif. In K+ solution, this sequence forms a propeller-type parallel-stranded G-quadruplex involving a 9-nt central double-chain-reversal loop. This long loop is anchored to the 5'-end of the sequence by an AT Watson Crick base pair and a potential G.A. noncanonical base pair. These base pairs contribute to the stability of the overall G-quadruplex structure, as measured by an increase of about 17 kcal/mol in enthalpy or 6 degrees C in melting temperature. Further, we demonstrate that such a monomorphic structure is formed within longer sequence contexts folding into a pearl-necklace structure.
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