Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 134, Issue 21, Pages 9006-9011Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja3033058
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Funding
- National Institutes of Health [R01 GM066041]
- National Science Foundation [DMR 0654118]
- State of Florida [DMR 0654118]
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The complex metabolic makeup of a biological system, such as a cell, is a key determinant of its biological state providing unique insights into its function. Here we characterize the metabolome of a cell by a novel homonuclear C-13 2D NMR approach applied to a nonfractionated uniformly C-13-enriched lysate of E. coli cells and determine de novo their carbon backbone topologies that constitute the topolome. A protocol was developed, which first identifies traces in a constant-time C-13-C-13 TOCSY NMR spectrum that are unique for individual mixture components and then assembles for each trace the corresponding carbon-bond topology network by consensus clustering. This led to the determination of 112 topologies of unique metabolites from a single sample. The topolome is dominated by carbon topologies of carbohydrates (34.8%) and amino acids (45.5%) that can constitute building blocks of more complex structures.
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