Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 134, Issue 17, Pages 7400-7405Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja302621v
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Funding
- National Science Foundation [CHE 1011957]
- National Science Foundation of China [20935002, 21075006, 20975043]
- Beijing Natural Science Foundation [2112003]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1011957] Funding Source: National Science Foundation
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Pressure-induced transport of double-stranded DNA (dsDNA) from 10 base pairs (bp) to 1.9 mega base pairs (Mbp) confined in a 750-nm-radius capillary was studied using a hydrodynamic chromatographic technique and four distinct length regions (rod-like, free-coiled, constant mobility, and transition regions) were observed. The transport behavior varied closely with region changes. The rod-like region consisted of DNA shorter than the persistence length (similar to 150 bp) of dsDNA, and these molecules behaved like polymer rods. Free-coiled region consisted of DNA from similar to 150 bp to similar to 2 kilo base pairs (kbp), and the effective hydrodynamic radius R-HD of these DNA scaled to L-0.5 (L is the DNA length in kb), a characteristic property of freely coiled polymers. Constant mobility region consisted of DNA longer than similar to 100 kbp, and these DNA had a constant hydrodynamic mobility and could not be resolved Transition region existed between free coiled and constant mobility regions. The transport mechanism of DNA in this region was complicated, and a general empirical equation was established to relate the mobility with DNA length. Understanding of the fundamental principles of DNA transport in narrow capillary channels will be of great interest in the development of lab-on-chip technologies and nongel DNA separations.
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