Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 133, Issue 2, Pages 365-372Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja106895k
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Funding
- NSFC [20874025]
- Program for New Century Excellent Talents in Universities [NCET-07-0688]
- National 973 Project [2007CB310500]
- National Science Foundation (NSF) Chemistry Division [CHE-0805547]
- Nanoscale Science and Engineering Initiative of NSF [EEC 0118007]
- Department of Defense [DAMD 17-03-1-0384, DoD CDMRP W81XWH-07-1-0428]
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Both photoswitchable fluorescent nanoparticles and photoactivatable fluorescent proteins have been used for super-resolution far-field imaging on the nanometer scale, but the photoactivating wavelength for such photochemical events generally falls in the near-UV (NUV) region (<420 nm), which is not preferred in cellular imaging. However, using two near-IR (NIR) photons that are lower in energy, we can circumvent such problems and replace NUV single-photon excitations (e.g., 390 nm) with NIR two-photon excitations (e.g., 780 nm). Thus, we have demonstrated that alternating 780 nm NIR two-photon and 488 nm single-photon excitations induces reversible on off fluorescence switching of immunotargeted nanoparticles in the human breast cancer cell line SK-BR-3. Herein, two-photon absorption not only caused spiropyran merocyanine photoisomerization within the particles but also imparted red fluorescence. In comparison with single-photon NUV excitations, two-photon NIR laser excitations can potentially reduce absorption-related photodamage to living systems because cellular systems absorb much more weakly in the NIR.
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