Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 133, Issue 7, Pages 2120-2123Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja110833r
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Funding
- DARPA US Army RDECOM Acquisition Center [W911NF-09-1-0069, W81XWH-08-1-0766]
- National Cancer Institute [U54CA151880, U54CA119341]
- Ryan Fellowship
- K.S.A. King Abdullah Scholarship
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We demonstrate that polyvalent DNA-functionalized gold nanoparticles (DNA-Au NPs) selectively enhance ribonuclease H (RNase H) activity while inhibiting most biologically relevant nucleases. This combination of properties is particularly interesting in the context of gene regulation, since high RNase H activity results in rapid mRNA degradation and general nuclease inhibition results in high biological stability. We have investigated the mechanism of selective RNase H activation and found that the high DNA density of DNA-Au NPs is responsible for this unusual behavior. This work adds to our understanding of polyvalent DNA-Au NPs as gene regulation agents and suggests a new model for selectively controlling protein-nanoparticle interactions.
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