Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 133, Issue 36, Pages 14157-14159Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja205809h
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan [22000006, 21659204]
- Industrial Technology Development Organization (NEDO) of Japan
- Funding Program for Next Generation World-Leading Researchers [LS023]
- Inoue Foundation for Science
- Konica Minolta Science and Technology Foundation
- Asahi Glass Foundation
- Grants-in-Aid for Scientific Research [22000006, 21659204] Funding Source: KAKEN
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To improve optical imaging of Ca2+ and to make available a distinct color window for multicolor imaging, we designed and synthesized CaSiR-1, a far-red to near-infrared fluorescence probe for Ca2+, using Si-rhodamine (SiR) as the fluorophore and the well-known Ca2+ chelator BAPTA. This wavelength region is advantageous, affording higher tissue penetration, lower background autofluorescence, and lower phototoxicity in comparison with the UV to visible range. CaSiR-1 has a high fluorescence off/on ratio of over 1000. We demonstrate its usefulness for multicolor fluorescence imaging of action potentials (visualized as increases in intracellular Ca2+) in brain slices loaded with sulforhodamine 101 (red color; specific for astrocytes) that were prepared from transgenic mice in which some neurons expressed green fluorescent protein.
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