4.8 Article

Development of a Far-Red to Near-Infrared Fluorescence Probe for Calcium Ion and its Application to Multicolor Neuronal Imaging

Journal

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 133, Issue 36, Pages 14157-14159

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ja205809h

Keywords

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Funding

  1. Ministry of Education, Culture, Sports, Science and Technology of Japan [22000006, 21659204]
  2. Industrial Technology Development Organization (NEDO) of Japan
  3. Funding Program for Next Generation World-Leading Researchers [LS023]
  4. Inoue Foundation for Science
  5. Konica Minolta Science and Technology Foundation
  6. Asahi Glass Foundation
  7. Grants-in-Aid for Scientific Research [22000006, 21659204] Funding Source: KAKEN

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To improve optical imaging of Ca2+ and to make available a distinct color window for multicolor imaging, we designed and synthesized CaSiR-1, a far-red to near-infrared fluorescence probe for Ca2+, using Si-rhodamine (SiR) as the fluorophore and the well-known Ca2+ chelator BAPTA. This wavelength region is advantageous, affording higher tissue penetration, lower background autofluorescence, and lower phototoxicity in comparison with the UV to visible range. CaSiR-1 has a high fluorescence off/on ratio of over 1000. We demonstrate its usefulness for multicolor fluorescence imaging of action potentials (visualized as increases in intracellular Ca2+) in brain slices loaded with sulforhodamine 101 (red color; specific for astrocytes) that were prepared from transgenic mice in which some neurons expressed green fluorescent protein.

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