Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 132, Issue 32, Pages 11036-11038Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja1052544
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Funding
- NSFC [20844003, 20834003, 20640420622]
- SRF for ROCS (SEM)
- NSF
- NSF DMR
- DOE-BES
- ARO
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RNA-coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA-protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid-protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.
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