Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 132, Issue 29, Pages 9937-9939Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja104702q
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Funding
- Ministry of Education, Science, Sports, Culture and Technology, Japan [18001001, 20750126]
- Global COE Program for Chemistry Innovation
- Association for the Progress of New Chemistry
- Grants-in-Aid for Scientific Research [20750126] Funding Source: KAKEN
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Desired enzyme nanoarrays patterned on a DNA origami scaffold were selectively isolated by affinity tag purification from a pool of differently patterned nanoarrays, and their enzymatic activity was successfully confirmed. As few as 12 histidine residues were enough to hold a huge complex of DNA origami with multiple proteins, 260 nm in length and 5.2 MDa in molecular weight, to an immobilized metal affinity resin.
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