Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 131, Issue 44, Pages 16021-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja904138v
Keywords
-
Categories
Funding
- National Institutes of Health [GM068122]
- Roche Research Foundation
- Gerhard Casper Fellowship
Ask authors/readers for more resources
RNA-templated fluorescence activation is a nucleic acid detection strategy that offers the possibility of direct visual detection of genetic information in living cells. Here we describe a new reaction strategy for fluorescence activation in which a phosphine on one DNA probe reduces an azide group in a linker on a second probe, resulting in linker cleavage and release of a fluorescence quenching group. These Q-STAR probes are shown to yield a strong fluorescence turn-on signal in similar to 20 min, with very tow background and substantial amplification by turnover on the template. A green/red pair of such probes allowed the discrimination of two bacterial species by a single nucleotide difference in their 16S rRNA. The beneficial properties of the reductive quencher release design make these probes promising candidates for widespread application in the detection of nucleic acids in vitro and in cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available