Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 131, Issue 20, Pages 7175-7181Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja901307m
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Funding
- National Institutes of Health [GM27681]
- Caltech Kanel predoctoral fellowship
- California TobaccoRelated Disease Research Program [16FT-0055]
- The National Science Foundation Chemistry Research Inst umentation and Facilities Program [CHE-0541745]
- UPLC-MS instrument
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Pyrrole-Imidazole polyamides are DNA-binding molecules that are programmable for a large repertoire of DNA sequences. Typical syntheses of this class of heterocyclic oligomers rely on solid-phase methods. Solid-phase methodologies offer rapid assembly on a micromole scale sufficient for biophysical characterizations and cell culture studies. In order to produce gram-scale quantities necessary for efficacy studies in animals, polyamides must be readily synthesized in solution. An 8-ring hairpin polyamide 1, which targets the DNA sequence 5'-WGWWCW-3', was chosen for our synthesis studies as this oligomer exhibits androgen receptor antagonism in cell culture models of prostate cancer. A convergent solution-phase synthesis of 1 from a small set of commercially available building blocks is presented which highlights principles for preparing gram quantities of pyrrole-imidazole oligomers with minimal chromatography.
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