Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 131, Issue 41, Pages 14624-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja9062426
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Funding
- National Natural Science Foundation [20627005]
- National Special Fund for SKLBE [2060204]
- Program for New Century Excellent Talents [NCET-07-0287]
- Shanghai Shuguang Program [09JC1404100, 06SG32]
- U S NIH
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An effective dual-DNAzyme-based unimolecular probe design employing intramolecular signal transduction is demonstrated. The probe is composed of three domains: a DNA-cleaving DNAzyme, a substrate, and an HRP-mimicking DNAzyme. When the probe meets its target, cleavage of the substrate by the DNA-cleaving DNAzyme activates the HRP-mimicking DNAzyme, producing a colorimetric signal. The Cu2+-dependent DNAzyme engineered to demonstrate this design revealed a sensitivity corresponding to 65 ppb, which is sufficient to detect Cu2+ in drinking water. The new probe has excellent selectivity toward Cu2+. This three-component design is simple and easy to engineer. It may provide the basis for future development of other nucleic acid-based probes for toxicological and environmental monitoring.
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