4.8 Article

Biosynthesis of the Thiamin Thiazole in Bacillus subtilis: Identification of the Product of the Thiazole Synthase-Catalyzed Reaction

Journal

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 131, Issue 9, Pages 3225-3229

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ja806752h

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Funding

  1. National Institutes of Health [DK44083]

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In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.

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