Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 131, Issue 21, Pages 7234-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja9026924
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- NIH [GM074785]
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The dioxygenation of nitric oxide by oxyheme in globin proteins is a major route for NO detoxification in aerobic biological. systems. In myoglobin, this reaction is thought to proceed through an iron(III)-bound peroxynitrite before homolytic cleavage of the O-O bond to form an iron(IV)-oxo and NO2 radical followed by recombination and nitrate production. Single turnover experiments at alkaline pH have revealed the presence of a millisecond high-spin heme intermediate. It is widely presumed that this species is an iron (III)-peroxynitrite species, but detailed characterization of the intermediate is lacking. Using resonance Raman spectroscopy and rapid-freeze quench techniques, we identify the Millisecond intermediate as an iron(III)-nitrato complex with a symmetric NO2 stretch at 1282 cm(-1). Greater time resolution techniques will be required to detect the putative iron(III) peroxynitrite complex.
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