4.8 Article

Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp

Journal

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 130, Issue 3, Pages 784-+

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ja0759139

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Funding

  1. Intramural NIH HHS Funding Source: Medline

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We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.

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