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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 130, Issue 7, Pages 2146-+Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja078051k
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Wet stamping initiated from a hydrogel reservoir soaked in a salt precipitant sets-up multiple conditions for the growth of protein crystals over an array of nanoliter wells. Diffusive delivery of the precipitant translates into time- and space-varying fluxes of the precipitant in the wells. These fluxes, in turn, control the numbers of seeded crystals as well as their dimensions. Reaction-diffusion equations with a nucleation term are developed to model the process of crystal growth and to relate the 'schedules' of precipitant delivery to the dimensions and habits of the protein microcrystals in the array.
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