Small-angle solution scattering using the mixed-mode pixel array detector

Solution small-angle X-ray scattering (SAXS) measurements were obtained using a 128 x 128 pixel X-ray mixed-mode pixel array detector (MMPAD) with an 860 mu s readout time. The MMPAD offers advantages for SAXS experiments: a pixel full-well of > 2 x 107 10 keV X-rays, a maximum flux rate of 108 X-rays pixel-1 s-1, and a sub-pixel point-spread function. Data from the MMPAD were quantitatively compared with data from a charge-coupled device (CCD) fiber-optically coupled to a phosphor screen. MMPAD solution SAXS data from lysozyme solutions were of equal or better quality than data captured by the CCD. The read-noise (normalized by pixel area) of the MMPAD was less than that of the CCD by an average factor of 3.0. Short sample-to-detector distances were required owing to the small MMPAD area (19.2 mm x 19.2 mm), and were revealed to be advantageous with respect to detector read-noise. As predicted by the Shannon sampling theory and confirmed by the acquisition of lysozyme solution SAXS curves, the MMPAD at short distances is capable of sufficiently sampling a solution SAXS curve for protein shape analysis. The readout speed of the MMPAD was demonstrated by continuously monitoring lysozyme sample evolution as radiation damage accumulated. These experiments prove that a small suitably configured MMPAD is appropriate for time-resolved solution scattering measurements.