Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 15, Issue -, Pages 420-422Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S0909049508009606
Keywords
protein denaturation; radiation damage; synchrotron radiation circular dichroism spectroscopy; photon flux; vacuum ultraviolet wavelengths
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Funding
- Biotechnology and Biological Sciences Research Council [BB/F010362/1] Funding Source: Medline
- BBSRC [BB/F010362/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F010362/1] Funding Source: researchfish
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New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.
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