Journal
JOURNAL OF SURGICAL RESEARCH
Volume 169, Issue 1, Pages 156-161Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2009.08.017
Keywords
tissue oxygen tension; latissimus dorsi muscle; tissue oxygenation; ischemia; reperfusion; cellular oxygen; tissue viability; near-infrared spectroscopy; rabbits
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Background. There is currently no efficient and reliable clinical means of assessing the degree of ischemia-and reperfusion-associated damage in microvascular transplants. The objective was to study correlation of tissue oxygen tension measurements with tissue oxygen saturation, cytochrome oxidase redox state, and tissue viability. Materials and Methods. Latissimus dorsi muscle was dissected and mobilized in New Zealand white rabbits (n = 30, 2.5 +/- 0.5 kg). Muscles were exposed to warm ischemia in two groups with either 4 or 6 h, followed by reperfusion. Tissue PO(2) was measured with a miniature intramuscular oxygen sensor (Licox microprobe; Integra Neurosciences, Ratingen, Germany) all along with tissue hemoglobin saturation (rSO(2)) and cytochrome oxidase aa3 redox state (CytOx) by in vivo near-infrared spectroscopy. Linear correlation was performed between tissue PO(2) and rSO(2), CytOx and tissue viability. Results. After ischemia and reperfusion, tissue PO(2) and CytOx recovery was significantly decreased in both groups compared with control (4 h: P < 0.05; 6 h: P < 0.01). Significant correlations between changes in tissue PO(2) and rSO(2) (r = 0.92; P < 0.01), CytOx (r = 0.90; P < 0.01), wet-to-dry ratio (r = -0.97; P < 0.01), and mitochondrial viability index (r = -0.97; P < 0.01) were found. Conclusions. Tissue oxygen tension measured with microprobes correlated closely with tissue oxygenation, cellular oxygen utilization, and the extent of ischemia reperfusion injury. (C) 2011 Elsevier Inc. All rights reserved.
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