Journal
JOURNAL OF SURGICAL RESEARCH
Volume 171, Issue 2, Pages E215-E221Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jss.2011.08.012
Keywords
pioglitazone; peroxisome proliferator-activated receptor-gamma; cecal ligation and puncture; adiponectin; omental adipocyte function
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Funding
- Japan Society for the Promotion of Science [20591537]
- Grants-in-Aid for Scientific Research [20591537, 23591880] Funding Source: KAKEN
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Background. To examine the effects of pioglitazone, peroxisome proliferator-activated receptor-gamma (PPAR-gamma), on mortality and omental adipocyte function in mice with cecal ligation and puncture (CLP). Methods. Male mice were assigned to receive (1) vehicle/sham-operation, (2) pioglitazone/CLP, or (3) vehicle/CLP. Pioglitazone was injected intraperitoneally for 7 d before operation. Serum and omental tissue were collected before, 24, and 48 h after CLP. Serum levels of adiponectin, cytokine, and chemokine were measured with ELISA. mRNA expressions in omental tissues were determined by RT-PCR. Survival was monitored for 7 d after CLP. Results. Survival after CLP was significantly better in the pioglitazone/CLP than in the vehicle/CLP. Serum adiponectin levels before CLP were higher in the pioglitazone/CLP than in the vehicle/CLP. Treatment with pioglitazone significantly inhibited the increases in the serum interleukin-6 and monocyte chemoattractant protein-1 (MCP-1) levels after CLP and lowered the mRNA expressions of proinflammatory cytokines, interleukin-6, and MCP-1 in omental tissue after CLP. Conclusion. The anti-inflammatory effects of pioglitazone on omental adipocyte function appear to be mediated in part by PPAR-gamma activation, which down-regulates the production of inflammatory mediators. (c) 2011 Elsevier Inc. All rights reserved.
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