Sentinel node micrometastases have high proliferative potential in gastric cancer

Background. The 6th edition of the TNM classification has recently defined sentinel nodes (SN), micrometastasis, and isolated tumor cells (ITC). The present study examines the frequency and proliferative activity of such metastases with focus on the SNs of gastric cancer. Methods. We enrolled 133 patients with cT1-2 tumors (cT1: 104, cT2: 29) and mapped SNs. Lymph node metastases were examined by routine histology and by immunohistochemistry with anti-cytokeratin. We used the Ki-67 antibody to detect the primary tumor and lymph node metastases to evaluate proliferative activity. Results. The number of patients with SNs metastases and metastatic SNs was 19 and 52, respectively. The frequencies of macrometastasis, micrometastasis, and ITC were 48%, 25%, and 27%, respectively. Ki-67 expression in the tumor closely correlated with lymphatic invasion (P = 0.0001), venous invasion (P < 0.0001), and lymph node metastasis (P < 0.0001). Cells in 96% of macrometastases, 92% of micrometastases, and 29%, of ITCs were Ki-67 positive. Conclusions. We showed that micrometastasis and some ITCs in SNs had proliferative activity. We suggest that micrometastasis and ITCs should be removed, especially during SN navigation surgery, until their clinical significance is clarified. (C) 2008 Elsevier Inc. All rights reserved.