Journal
JOURNAL OF SUPERCRITICAL FLUIDS
Volume 95, Issue -, Pages 8-16Publisher
ELSEVIER
DOI: 10.1016/j.supflu.2014.07.025
Keywords
Extraction; Antioxidant; Anthocyanins; Blueberry; Food wastes
Categories
Funding
- CAPES
- CNPq [473342/2011-1]
- FAPESP [2013/02203-6]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/02203-6] Funding Source: FAPESP
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This work explored the potential of subcritical liquids and supercritical carbon dioxide (CO2) in the recovery of extracts containing phenolic compounds, antioxidants and anthocyanins from residues of blueberry (Vaccinium myrtillus L.) processing. Supercritical CO2 and pressurized liquids are alternatives to the use of toxic organic solvents or extraction methods that apply high temperatures. Blueberry is the fruit with the highest antioxidant and polyphenol content, which is present in both peel and pulp. In the extraction with pressurized liquids (PLE), water, ethanol and acetone were used at different proportions, with temperature, pressure and solvent flow rate kept constant at 40 degrees C, 20 MPa and 10 ml/min, respectively. The extracts were analyzed and the highest antioxidant activities and phenolic contents were found in the extracts obtained with pure ethanol and ethanol + water. The highest concentrations of anthocyanins were recovered with acidified water as solvent. In supercritical fluid extraction (SFE) with CO2, water, acidified water, and ethanol were used as modifiers, and the best condition for all functional components evaluated was SFE with 90% CO2, 5% water, and 5% ethanol. Sixteen anthocyanins were identified and quantified by ultra performance liquid chromatography (UPLC). (C) 2014 Elsevier B.V. All rights reserved.
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