Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 171, Issue 2, Pages 216-222Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2010.03.017
Keywords
Asymmetric reconstruction; Cryo-electron microscopy; Starting model; Model validation; Single-particle reconstruction; Symmetry determination
Funding
- National Institute of Allergy and Infectious Diseases [R21A1081221]
- BYU
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Model-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models directly from un-averaged, experimental images of asymmetric or symmetric particles. Therefore, the asymmetric RMM can also be used to determine point-group symmetry. The procedure is facilitated by the use of (a) variable angular step-sizes during iterative origin and orientation searches, (b) high numbers of particle images, and (c) highly defocused images. The method is inhibited by mixed-handedness orientation assignments and by particles with inconspicuous features. For symmetric particles, symmetric RMMs can overcome these deficiencies. (C) 2010 Elsevier Inc. All rights reserved.
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