Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 168, Issue 1, Pages 200-206Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2009.07.008
Keywords
Supported lipid bilayer; Quartz crystal microbalance; Membrane junction; DNA
Funding
- INGVAR
- Swedish Strategic Research Foundation
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Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture. (C) 2009 Elsevier Inc. All rights reserved.
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