Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 168, Issue 1, Pages 95-106Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2009.02.013
Keywords
Fluorescence microscopy; Optical microscopy; Evanescent; Fluctuation; Single molecule; Surface binding; Diffusion; Supported membranes; Cell membranes
Funding
- NSF [MCB-0641087]
- NIH [GM-041402]
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In this paper, the conceptual basis and experimental design of total internal reflection with fluorescence correlation spectroscopy (TIR-FCS) is described. The few applications to date of TIR-FCS to supported membranes are discussed, in addition to a variety of applications not directly involving supported membranes. Methods related, but not technically equivalent, to TIR-FCS are also summarized. Future directions for TIR-FCS are outlined. (C) 2009 Elsevier Inc. All rights reserved.
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