4.8 Article

The Rice High-Affinity Potassium Transporter1;1 Is Involved in Salt Tolerance and Regulated by an MYB-Type Transcription Factor

Journal

PLANT PHYSIOLOGY
Volume 168, Issue 3, Pages 1076-+

Publisher

OXFORD UNIV PRESS INC
DOI: 10.1104/pp.15.00298

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Funding

  1. Ministry of Science and Technology in China [2012CB114200]
  2. National Natural Science Foundation of China [31171461, 91117003, 31301294]
  3. Fundamental Research Funds for the Central Universities [KYTZ201402]
  4. Priority Academic Program Development of Jiangsu Higher Education Institutions

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Sodium transporters play key roles in plant tolerance to salt stress. Here, we report that a member of the High-Affinity K+ Transporter (HKT) family, OsHKT1;1, in rice (Oryza sativa 'Nipponbare') plays an important role in reducing Na+ accumulation in shoots to cope with salt stress. The oshkt1;1 mutant plants displayed hypersensitivity to salt stress. They contained less Na+ in the phloem sap and accumulated more Na+ in the shoots compared with the wild type. OsHKT1; 1 was expressed mainly in the phloem of leaf blades and up-regulated in response to salt stress. Using a yeast one-hybrid approach, a novel MYB coiled-coil type transcription factor, OsMYBc, was found to bind to the OsHKT1; 1 promoter. In vivo chromatin immunoprecipitation and in vitro electrophoresis mobility shift assays demonstrated that OsMYBc binds to AAANATNC(C/T) fragments within the OsHKT1; 1 promoter. Mutation of the OsMYBc-binding nucleotides resulted in a decrease in promoter activity of OsHKT1;1. Knockout of OsMYBc resulted in a reduction in NaCl-induced expression of OsHKT1; 1 and salt sensitivity. Taken together, these results suggest that OsHKT1; 1 has a role in controlling Na+ concentration and preventing sodium toxicity in leaf blades and is regulated by the OsMYBc transcription factor.

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