Journal
PLANT PHYSIOLOGY
Volume 168, Issue 2, Pages 752-+Publisher
OXFORD UNIV PRESS INC
DOI: 10.1104/pp.15.00319
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Funding
- Japan Society for the Promotion of Science [25850068, 25120714]
- Advanced Low Carbon Technology Research and Development Program of the Japan Science and Technology Agency
- Core Research for Evolutional Science and Technology Program of the JST
- Grants-in-Aid for Scientific Research [25850068, 15K18682, 25113503] Funding Source: KAKEN
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Although microalgae accumulate triacylglycerol (TAG) and starch in response to nutrient-deficient conditions, the regulatory mechanisms are poorly understood. We report here the identification and characterization of a kinase, triacylglycerol accumulation regulator1 (TAR1), that is a member of the yeast (Saccharomyces cerevisiae) Yet another kinase1 (Yak1) subfamily in the dual-specificity tyrosine phosphorylation-regulated kinase family in a green alga (Chlamydomonas reinhardtii). The kinase domain of TAR1 showed auto-and transphosphorylation activities. A TAR1-defective mutant, tar1-1, accumulated TAG to levels 0.5- and 0.1-fold of those of a wild-type strain in sulfur (S)- and nitrogen (N)-deficient conditions, respectively. In N-deficient conditions, tar1-1 showed more pronounced arrest of cell division than the wild type, had increased cell size and cell dry weight, and maintained chlorophyll and photosynthetic activity, which were not observed in S-deficient conditions. In N-deficient conditions, global changes in expression levels of N deficiency-responsive genes in N assimilation and tetrapyrrole metabolism were noted between tar1-1 and wild-type cells. These results indicated that TAR1 is a regulator of TAG accumulation in S-and N-deficient conditions, and it functions in cell growth and repression of photosynthesis in conditions of N deficiency.
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