Journal
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
Volume 113, Issue 3-5, Pages 275-280Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jsbmb.2009.01.011
Keywords
Tanshinone IIA; Estrogen-like; Phytoestrogen; MCF-7 cells; MDA-MB-231 cells; RAW 264.7 cells; inflammation
Funding
- Technology Development Plan of Tianjin [06TXTJJC13600]
- Research Fund for the Doctoral Program of Higher Education (RFDP), China [20060063002]
- National Program for Key Basic Research Projects [2005CB523404]
- International Cooperative Project of Science and Technology Ministry [2008DFB30070]
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Tanshinone IIA (Tan IIA) is a major compound extracted from a traditional herbal medicine Salvia miltiorrhiza BUNGE, which is used to treat cardiovascular diseases, cerebrovascular diseases and postmenopausal syndrome, it has also been shown to possess anti-inflammatory activity. Since Tan IIA has a similar structure to that of 17 beta-estradiol (E-2), the present study was undertaken to characterize the estrogenic activity of Tan IIA and to demonstrate a functional role of this activity in RAW 264.7 cells. In transient transfection assay, Tan IIA (10 mu M) increases ERE-luciferase activity in an estrogen receptor (ER) subtype-dependent manner when either ER alpha or ER beta were co-expressed in Hela cells. In LPS-induced RAW 264.7 cells, Tan IIA exerts anti-inflammatory effects by inhibition of iNOS gene expression and NO production, as well as inhibition of inflammatory cytokine (IL-1 beta, IL-6, and TNF-alpha) expression via ER-dependent pathway. Therefore, it could serve as a potential selective estrogen receptor modulator (SERM) to treat inflammation-associated neurodegenerative and cardiovascular diseases without increasing the risk of breast cancer. (C) 2009 Elsevier Ltd. All rights reserved.
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