Journal
PLANT PHYSIOLOGY
Volume 168, Issue 3, Pages 1140-+Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1104/pp.15.00580
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Funding
- European Union Marie-Curie Initial Training Network (Initial Training Network Croplife Project) [PITN-GA-2010-264394]
- Biological Sciences Research Council, UK [BB/M009130/1]
- Rural and Environment Science and Analytical Services Division of the Scottish Government
- Biotechnology and Biological Sciences Research Council [BB/M009130/1] Funding Source: researchfish
- BBSRC [BB/M009130/1] Funding Source: UKRI
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WHIRLY1 is largely targeted to plastids, where it is a major constituent of the nucleoids. To explore WHIRLY1 functions in barley (Hordeum vulgare), RNA interference-knockdown lines (W1-1, W1-7, and W1-9) that have very low levels of HvWHIRLY1 transcripts were characterized in plants grown under optimal and stress conditions. The WHIRLY1-1 (W1-1), W1-7, and W1-9 plants were phenotypically similar to the wild type but produced fewer tillers and seeds. Photosynthesis rates were similar in all lines, but W1-1, W1-7, and W1-9 leaves had significantly more chlorophyll and less sucrose than the wild type. Transcripts encoding specific subsets of chloroplast-localized proteins, such as ribosomal proteins, subunits of the RNA polymerase, and thylakoid nicotinamide adenine dinucleotide (reduced) and cytochrome b(6)/f complexes, were much more abundant in the W1-7 leaves than the wild type. Although susceptibility of aphid (Myzus persicae) infestation was similar in all lines, the WHIRLY1-deficient plants showed altered responses to nitrogen deficiency, maintaining higher photosynthetic CO2 assimilation rates than the wild type under limiting nitrogen. Although all lines showed globally similar low nitrogen-dependent changes in transcripts and metabolites, the increased abundance of FAR-RED IMPAIRED RESPONSE1-like transcripts in nitrogen-deficient W1-7 leaves infers that WHIRLY1 has a role in communication between plastid and nuclear genes encoding photosynthetic proteins during abiotic stress.
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