Journal
PLANT PATHOLOGY JOURNAL
Volume 31, Issue 4, Pages 438-440Publisher
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.NT.06.2015.0110
Keywords
LAMP; loop-mediated isothermal amplification; quarantine; wheat streak mosaic virus; WSMV
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Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2009-0093829]
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We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.
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