Journal
PLANT MOLECULAR BIOLOGY REPORTER
Volume 33, Issue 6, Pages 1967-1974Publisher
SPRINGER
DOI: 10.1007/s11105-015-0888-4
Keywords
Aphid; (E)-beta-farnesene synthase; Oryza sativa; Rhopalosiphum padi; Transgenic rice
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Funding
- China-UK Programme in Global Priorities [2013DFG32230, BB/L001683/1]
- Special Fund for Agro-scientific Research in the Public Interest of China [201403030]
- Biotechnology and Biological Sciences Research Council (BBSRC) International Partnering Award [BB/J02028/1]
- Biotechnology and Biological Sciences Research Council [BB/L001683/1] Funding Source: researchfish
- BBSRC [BB/L001683/1] Funding Source: UKRI
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Aphids (Aphididae) cause great damage to economically important cereal crops due to their unparalleled reproductive capacity. The sesquiterpene (E)-beta-farnesene (E beta F) is the alarm pheromone for many aphids, which can be applied to disperse aphids in crops. The peppermint E beta F synthase gene (E beta FS), which enables the production of E beta F in plants to control aphids, has been used in Arabidopsis and tobacco, but has not been studied in rice or other crops yet. Here, we transformed the peppermint E beta FS gene into the rice variety Zhonghua 11 and obtained two homozygous transgenic lines with single-copy insertion. E beta FS expression and E beta F production were then verified by reverse transcription-PCR (RT-PCR)/quantitative real-time PCR (qPCR) and gas chromatography-mass spectrometry (GC-MS). Although only a limited amount of E beta F was detected, the transgenic lines showed a significant repelling effect on bird cherry-oat aphid (Rhopalosiphum padi) compared with the control. Thus, this study provides a valuable reference for improving the resistance against aphids in rice or other important crops.
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