Journal
PLANT JOURNAL
Volume 82, Issue 4, Pages 669-679Publisher
WILEY
DOI: 10.1111/tpj.12841
Keywords
jasmonate; JAsmonate-ZIM; TIFY protein; transcription; hormone signaling; lineage-specific gene; Arabidopsis thaliana
Categories
Funding
- National Institutes of Health [R01GM57795, R01GM57795-11S1]
- Chemical Sciences, Geosciences and Biosciences Division, Office of Basic Energy Sciences, Office of Science, U.S. Department of Energy [DE-FG02-91ER20021]
- Japan Society for the Promotion of Science
- Michigan AgBioResearch Project [MICL02278]
- Discretionary Research Initiative from Michigan State University
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JAsmonate ZIM-domain (JAZ) proteins repress the activity of transcription factors that execute responses to the plant hormone jasmonoyl-L-isoleucine (JA-Ile). The ZIM protein domain recruits the co-repressors NINJA and TOPLESS to JAZ-bound transcription factors, and contains a highly conserved TIF[F/Y]XG motif that defines the larger family of TIFY proteins to which JAZs belong. Here, we report that diverse plant species contain genes encoding putative non-TIFY JAZ proteins, including a previously unrecognized JAZ repressor in Arabidopsis (JAZ13, encoded by At3g22275). JAZ13 is most closely related to JAZ8 and includes divergent EAR, TIFY/ZIM, and Jas motifs. Unlike JAZ8, however, JAZ13 contains a Ser-rich C-terminal tail that is a site for phosphorylation. Overexpression of JAZ13 resulted in reduced sensitivity to JA, attenuation of wound-induced expression of JA-response genes, and decreased resistance to insect herbivory. JAZ13 interacts with the bHLH transcription factor MYC2 and the co-repressor TOPLESS but, consistent with the absence of a TIFY motif, neither NINJA nor other JAZs. Analysis of single and higher-order T-DNA insertion jaz null mutants provided further evidence that JAZ13 is a repressor JA signaling. Our results demonstrate that proteins outside the TIFY family are functional JAZ repressors and further suggest that this expansion of the JAZ family allows fine-tuning of JA-mediated transcriptional responses. Significance Statement The research shows that the repertoire of JAZ repressors includes proteins outside the TIFY family. The existence of non-TIFY JAZs provides new insight into the mechanism of jasmonate-mediated transcriptional control and also how JAZs arise and acquire functionality during evolution.
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