Journal
PLANT JOURNAL
Volume 84, Issue 3, Pages 611-620Publisher
WILEY
DOI: 10.1111/tpj.13015
Keywords
long-distance; xylem sap; peptidomics; CLV3/ESR-related; C-terminally encoded peptide; sulfated peptide
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Funding
- NIBB
- Japan Society for the Promotion of Science (JSPS) [A2406127, 15K18553]
- Ministry of Education, Culture, Sports, Science and Technology [22128006, 25221105]
- MEXT [15H05957]
- Grants-in-Aid for Scientific Research [15H05957, 15K18553] Funding Source: KAKEN
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There is a growing awareness that secreted peptides mediate organ-to-organ communication in higher plants. Xylem sap peptidomics is an effective but challenging approach for identifying long-distance mobile peptides. In this study we developed a simple, gel-free purification system that combines o-chlorophenol extraction with HPLC separation. Using this system, we successfully identified seven oligopeptides from soybean xylem sap exudate that had one or more post-transcriptional modifications: glycosylation, sulfation and/or hydroxylation. RNA sequencing and quantitative PCR analyses showed that the peptide-encoding genes are expressed in multiple tissues. We further analyzed the long-distance translocation of four of the seven peptides using gene-encoding peptides with single amino acid substitutions, and identified these four peptides as potential root-to-shoot mobile oligopeptides. Promoter-GUS analysis showed that all four peptide-encoding genes were expressed in the inner tissues of the root endodermis. Moreover, we found that some of these peptide-encoding genes responded to biotic and/or abiotic factors. These results indicate that our purification system provides a comprehensive approach for effectively identifying endogenous small peptides and reinforce the concept that higher plants employ various peptides in root-to-shoot signaling.
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