Journal
PLANT JOURNAL
Volume 82, Issue 1, Pages 138-150Publisher
WILEY-BLACKWELL
DOI: 10.1111/tpj.12799
Keywords
heterotrimeric G protein; ethylene; stomatal closure; H2O2; G subunit; NADPH oxidases; ethylene receptor; signalling; Arabidopsis thaliana
Categories
Funding
- National Science Foundation of China [31170370]
- Fundamental Research Funds for the Central Universities [GK201401005]
- Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education [ZS14004]
- Undergraduate Students Innovative Experimental Projects of Shaanxi Normal University [201310718017]
Ask authors/readers for more resources
Heterotrimeric G proteins function as key players in hydrogen peroxide (H2O2) production in plant cells, but whether G proteins mediate ethylene-induced H2O2 production and stomatal closure are not clear. Here, evidences are provided to show the G subunit GPA1 as a missing link between ethylene and H2O2 in guard cell ethylene signalling. In wild-type leaves, ethylene-triggered H2O2 synthesis and stomatal closure were dependent on activation of G. GPA1 mutants showed the defect of ethylene-induced H2O2 production and stomatal closure, whereas wG and cG overexpression lines showed faster stomatal closure and H2O2 production in response to ethylene. Ethylene-triggered H2O2 generation and stomatal closure were impaired in RAN1, ETR1, ERS1 and EIN4 mutants but not impaired in ETR2 and ERS2 mutants. G activator and H2O2 rescued the defect of RAN1 and EIN4 mutants or etr1-3 in ethylene-induced H2O2 production and stomatal closure, but only rescued the defect of ERS1 mutants or etr1-1 and etr1-9 in ethylene-induced H2O2 production. Stomata of CTR1 mutants showed constitutive H2O2 production and stomatal closure, but which could be abolished by G inhibitor. Stomata of EIN2, EIN3 and ARR2 mutants did not close in responses to ethylene, G activator or H2O2, but do generate H2O2 following challenge of ethylene or G activator. The data indicate that G mediates ethylene-induced stomatal closure via H2O2 production, and acts downstream of RAN1, ETR1, ERS1, EIN4 and CTR1 and upstream of EIN2, EIN3 and ARR2. The data also show that ETR1 and ERS1 mediate both ethylene and H2O2 signalling in guard cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available