Journal
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
Volume 14, Issue 9, Pages 1681-1688Publisher
SPRINGER
DOI: 10.1007/s10008-010-1010-3
Keywords
MWNT-PAMAM-Chit nanocomposite; Cytochrome c; DNA; Nitrite biosensor; High reactive Cyt c pi-cation
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Funding
- National Natural Science Foundation of China [20775044]
- Natural Science Foundation of Shandong province, China [Y2006B20]
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A novel and sensitive biosensor was developed for the determination of nitrite. Firstly, multi-walled carbon nanotubes-poly(amidoamine)-chitosan (MWNT-PAMAM-Chit) nanocomposite along with the incorporation of DNA was used to modify the glassy carbon electrode. Then the immobilization of Cyt c was accomplished using electrochemical deposition method by consecutive cyclic voltammetry (CV) scanning in a neutral Cyt c solution. CV behaviors of the modified electrodes showed that the MWNT-PAMAM-Chit nanocomposite is a good platform for the immobilization of DNA and Cyt c in order, at the same time, an excellent promoter for the electron transfer between Cyt c and the electrode. At high potential, the immobilized Cyt c could be further oxidized into highly reactive Cyt c pi-cation by two-step electrochemical oxidation, which could oxidize NO2 (-) into NO3 (-) in the solution. Therefore, a nitrite biosensor based on the biocatalytic oxidation of the immobilized Cyt c was fabricated, which showed a fast response to nitrite (less than 5 s). The linear range of 0.2-80 mu M and a detection limit of 0.03 mu M was obtained. Finally, the application in food analysis using sausage as testing samples was also investigated.
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