Journal
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
Volume 14, Issue 6, Pages 975-980Publisher
SPRINGER
DOI: 10.1007/s10008-009-0895-1
Keywords
Poly(pyrrole-2-aminobenzoic acid); Conducting polymer; Enzyme electrode; SERS
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Funding
- Gazi University Research Fund [02/2007-06, 02/2005-20]
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Polymerization of pyrrole and 2-aminobenzoic acid has been investigated, and a functionalized stable film of poly(pyrrole-2-aminobenzoic acid) (PP2ABA) has been obtained electrochemically onto platinum electrode. Different cyclic voltammetric behavior is obtained for polypyrrole and PP2ABA during electrosynthesis. Fourier-transformed infrared spectrometry and surface-enhanced Raman spectrometry measurements on the two films have confirmed the presence of carboxylate group in the films. The enzyme, glucose oxidase, was covalently immobilized on a conducting PP2ABA film, and amperometric response was measured as a function of concentration of glucose at a potential of 0.7 V vs Ag/AgCl in 0.1 M phosphate buffer at pH 6.2. The effect of polymeric film thickness, pH, and possible interferents were investigated. The linear range of the calibration curve is from 3 to 40 mM with a sensitivity of 0.058 A mu A mM(-1) cm(-2) and a limit of detection of 0.5 mM. The apperent Mishaelis-Menten constant K (M) is calculated to be 1 x 10(-2) mM, and the response time is 5 s.
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