Journal
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
Volume 13, Issue 5, Pages 657-663Publisher
SPRINGER
DOI: 10.1007/s10008-008-0590-7
Keywords
Amperometric biosensor; Enzyme electrodes; Conducting polymers; Cholesterol; Cholesterol oxidase
Categories
Funding
- TUBA
- [DPT 2005K 120580]
- [TUBITAK 105T385]
- [BAP-2007-01-03-03]
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Two conducting polymers, poly(pyrrole) (PPy) and poly(3,4-ethylenedioxythiophene) (PEDOT) were used as immobilization matrices for cholesterol oxidase (ChOx). The amperometric responses of the enzyme electrodes were measured by monitoring oxidation current of H2O2 at +0.7 V in the absence of a mediator. Kinetic parameters, such as K (m) and I (max), operational and storage stabilities, effects of pH and temperature were determined for both entrapment supports. K (m) values are found as 7.9 and 1.3 mM for PPy and PEDOT enzyme electrodes, respectively; it can be interpreted that ChOx immobilized in PEDOT shows higher affinity towards the substrate.
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