Journal
PLANT CELL
Volume 27, Issue 4, Pages 1265-1278Publisher
OXFORD UNIV PRESS INC
DOI: 10.1105/tpc.15.00058
Keywords
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Funding
- US National Science Foundation [IOS-1139329, PGRP-1026555]
- Japan Science and Technology Agency
- Science and Technology Star of Zhujiang, Guangzhou City [2013J2200082]
- Boyce Thompson Institute
- Grants-in-Aid for Scientific Research [24120006, 22380068] Funding Source: KAKEN
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Non-protein amino acids, often isomers of the standard 20 protein amino acids, have defense-related functions in many plant species. A targeted search for jasmonate-induced metabolites in cultivated rice (Oryza sativa) identified (R)-beta-tyrosine, an isomer of the common amino acid (S)-alpha-tyrosine in the seeds, leaves, roots, and root exudates of the Nipponbare cultivar. Assays with 119 diverse cultivars showed a distinct presence/absence polymorphism, with b-tyrosine being most prevalent in temperate japonica cultivars. Genetic mapping identified a candidate gene on chromosome 12, which was confirmed to encode a tyrosine aminomutase (TAM1) by transient expression in Nicotiana benthamiana and in vitro enzyme assays. A point mutation in TAM1 eliminated beta-tyrosine production in Nipponbare. Rice cultivars that do not produce b-tyrosine have a chromosome 12 deletion that encompasses TAM1. Although beta-tyrosine accumulation was induced by the plant defense signaling molecule jasmonic acid, bioassays with hemipteran and lepidopteran herbivores showed no negative effects at physiologically relevant beta-tyrosine concentrations. In contrast, root growth of Arabidopsis thaliana and other tested dicot plants was inhibited by concentrations as low as 1 mM. As beta-tyrosine is exuded into hydroponic medium at higher concentrations, it may contribute to the allelopathic potential of rice.
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