Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 13, Issue 8, Pages 1121-1135Publisher
WILEY
DOI: 10.1111/pbi.12412
Keywords
molecular farming; plant virus vectors; Agrobacterium; deconstructed viral vectors; agroinfiltration; recombinant protein expression in plants
Funding
- UK Biotechnological and Biological Sciences Research Council (BBSRC) [BB/J004596/1]
- John Innes Foundation
- Biotechnology and Biological Sciences Research Council [BBS/E/J/00000166, BB/L020955/1, BB/L014130/1, 982578] Funding Source: researchfish
- BBSRC [BB/L014130/1, BB/L020955/1] Funding Source: UKRI
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In the early days of molecular farming, Agrobacterium-mediated stable genetic transformation and the use of plant virus-based vectors were considered separate and competing technologies with complementary strengths and weaknesses. The demonstration that 'agroinfection' was the most efficient way of delivering virus-based vectors to their target plants blurred the distinction between the two technologies and permitted the development of 'deconstructed' vectors based on a number of plant viruses. The tobamoviruses, potexviruses, tobraviruses, geminiviruses and comoviruses have all been shown to be particularly well suited to the development of such vectors in dicotyledonous plants, while the development of equivalent vectors for use in monocotyledonous plants has lagged behind. Deconstructed viral vectors have proved extremely effective at the rapid, high-level production of a number of pharmaceutical proteins, some of which are currently undergoing clinical evaluation.
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