Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 13, Issue 7, Pages 983-992Publisher
WILEY
DOI: 10.1111/pbi.12341
Keywords
blackleg; Brassica napus; disease resistance; Leptosphaeria maculans; receptor-like protein; SOBIR1
Funding
- Western Grains Research Foundation
- SaskCanola
- Government of Saskatchewan Agricultural Development Fund
Ask authors/readers for more resources
Leucine-rich repeat receptor-like proteins (LRR-RLPs) are highly adaptable parts of the signalling apparatus for extracellular detection of plant pathogens. Resistance to blackleg disease of Brassica spp. caused by Leptosphaeria maculans is largely governed by host race-specific R-genes, including the LRR-RLP gene LepR3. The blackleg resistance gene Rlm2 was previously mapped to the same genetic interval as LepR3. In this study, the LepR3 locus of the Rlm2Brassica napus line Glacier DH24287' was cloned, and B.napus transformants were analysed for recovery of the Rlm2 phenotype. Multiple B.napus, B.rapa and B.juncea lines were assessed for sequence variation at the locus. Rlm2 was found to be an allelic variant of the LepR3 LRR-RLP locus, conveying race-specific resistance to L.maculans isolates harbouring AvrLm2. Several defence-related LRR-RLPs have previously been shown to associate with the RLK SOBIR1 to facilitate defence signalling. Bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation of RLM2-SOBIR1 studies revealed that RLM2 interacts with SOBIR1 of Arabidopsis thaliana when co-expressed in Nicotiana benthamiana. The interaction of RLM2 with AtSOBIR1 is suggestive of a conserved defence signalling pathway between B.napus and its close relative A.thaliana.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available