Journal
PLANT BIOTECHNOLOGY JOURNAL
Volume 14, Issue 2, Pages 783-790Publisher
WILEY
DOI: 10.1111/pbi.12429
Keywords
tomato; fatty acid desaturase; LeFAD7; RNA silencing; high-temperature tolerance; graft transmission
Funding
- Program for Promotion of Basic and Applied Researches in Bio-oriented Industry and Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry
- Ministry of Education, Culture, Sports, Science and Technology of Japan [24580065, 26292026]
- Japan Advanced Plant Science Network
- Grants-in-Aid for Scientific Research [24580065, 15K14664, 26292026] Funding Source: KAKEN
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We investigated graft transmission of high-temperature tolerance in tomato scions to nontransgenic scions from transgenic rootstocks, where the fatty acid desaturase gene (LeFAD7) was RNA-silenced. Tomato was transformed with a plasmid carrying an inverted repeat of LeFAD7 by Agrobacterium. Several transgenic lines showed the lower amounts of LeFAD7 RNA and unsaturated fatty acids, while nontransgenic control did not, and siRNA was detected in the transgenic lines, but not in control. These lines grew under conditions of high temperature, while nontransgenic control did not. Further, the nontransgenic plants were grafted onto the silenced transgenic plants. The scions showed less of the target gene RNA, and siRNA was detected. Under high-temperature conditions, these grafted plants grew, while control grafted plants did not. Thus, it was shown that high-temperature tolerance was conferred in the nontransgenic scions after grafting onto the silenced rootstocks.
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