Journal
PLANT BIOTECHNOLOGY
Volume 32, Issue 4, Pages 309-U156Publisher
JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
DOI: 10.5511/plantbiotechnology.15.1103a
Keywords
Alcohol dehydrogenase gene 5 '-untranslated region; omega; phylogeny; tobacco etch virus leader; translational enhancer
Funding
- Development of Fundamental Technologies for the Production of High-Value Materials Using Transgenic Plants project
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Translational enhancers are effective tools to increase the expression level of transgenes in plant cells, and some candidate elements are in use today. However, knowledge about suitable elements for a given plant species are limited. We aim here to make a catalogue of translational enhancers, and evaluated the effectiveness of 5'-UTRs of Nicotiana tabacum alcohol dehysrogenase gene (NtADH 5'-UTR), Arabidopsis thaliana ADH gene (AtADH 5'-UTR), Oryza sativa ADH gene (OsADH 5'-UTR), O derived from tobacco mosaic virus, and tobacco etch virus leader (TEVL) in various plant species. We found that the OsADH 5'-UTR is functional in all plant species tested, and that O and TEVL are effective in eudicots but not in monocots. From these results, we speculate that the degree of translational enhancement of any element in a given plant species is closely correlated with the phylogenic position of the species.
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