Journal
PLANT BIOLOGY
Volume 17, Issue 6, Pages 1176-1186Publisher
WILEY
DOI: 10.1111/plb.12344
Keywords
heavy metal-associated isoprenylated plant protein; Puccinia striiformis f; sp; Tritici; stress response; virus-induced gene silencing; wheat
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Funding
- National Basic Research Program of China [2013CB127700]
- National Natural Science Foundation of China [31401689, 31201463]
- Natural Science Foundation Research Project of Shaanxi Province [2015JQ3085]
- Basic Scientific Research Business Expenses [QN2013037]
- Ministry of Education of China [B07049]
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In cells, metallochaperones are important proteins that safely transport metal ions. Heavy metal-associated isoprenylated plant proteins (HIPPs) are metallochaperones that contain a metal binding domain and a CaaX isoprenylation motif at the carboxy-terminal end. To investigate the roles of wheat heavy metal-associated isoprenylated plant protein (TaHIPP) genes in plant development and in stress responses, we isolated cDNA encoding the wheat TaHIPP1 gene, which contains a heavy metal-associated domain, nuclear localisation signals and an isoprenylation motif (CaaX motif). Quantitative real-time PCR analysis indicated that the TaHIPP1 gene was differentially expressed under biotic and abiotic stresses. Specifically, TaHIPP1 expression was up-regulated by ABA exposure or wounding. Additionally, TaHIPP1 over-expression in yeast (Schizosaccharomyces pombe) significantly increased the cell growth rate under Cu2+ and high salinity stresses. The nuclear localisation of the protein was confirmed with confocal laser scanning microscopy of epidermal onion cells after particle bombardment with chimeric TaHIPP1-GFP constructs. In addition, TaHIPP1 was shown to enhance the susceptibility of wheat to Pst as determined by virus-induced gene silencing. These data indicate that TaHIPP1 is an important component in defence signalling pathways and may play a crucial role in the defence response of wheat to biotic and certain abiotic stresses.
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