Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 2, Pages 309-315Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201401115
Keywords
Atorvastatin; Blood analysis; Microextraction; Organic drop; Solidification
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Funding
- research council of Razi University
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A novel and sensitive dispersive liquid-liquid microextraction method based on the solidification of the floating organic drop combined with high-performance liquid chromatography and ultraviolet detection was used for the determination of atorvastatine in blood serum samples. The chromatographic separation of atorvastatin was carried out using methanol as the mobile phase organic modifier. Various parameters affecting the extraction efficiency were optimized, such as the kind and volume of extraction solvent (1-undecanol) and disperser solvent (acetonitrile), pH, and the extraction time. The calibration curve was linear in the range of 0.2-6000 mu g/L of atorvastatin (r(2) = 0.995) with a limit of detection of 0.07 mu g/L. The relative standard deviation for 100 mu g/L of atorvastatin in human plasma was 8.4% (n = 4). The recoveries of plasma samples spiked with atorvastatin were in the range of 98.8-113.8%. The obtained results showed that the proposed method is fast, simple, and reliable for the determination of very low concentrations of atorvastatin in human plasma samples.
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