Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 2, Pages 197-204Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201400955
Keywords
Beeswax; Liquid chromatography; Mass spectrometry; Piceid isomers; Resveratrol isomers
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Funding
- Spanish Ministry Educacion, Cultura y Deporte
- [RTA2012-00076-CO2-02]
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This paper represents the first report of a liquid chromatography coupled to electrospray ionization mass spectrometry method for simultaneously analyzing resveratrol and piceid isomers (cis and trans) in beeswax. An efficient extraction procedure has been proposed (average analyte recoveries were between 89 and 95%); this involved a solid-liquid extraction using a mixture of ethanol and water (80: 20, v/v) and a concentration step in a rotary evaporator. The separation of all the compounds was achieved using a C-18 column and a mobile phase composed of ammonium formate 0.03 M in water and acetonitrile in gradient elution mode at a flow rate of 1 mL/min. The method was fully validated in terms of selectivity, limits of detection and quantification, linearity, precision, and accuracy. The limits of detection and quantification ranged from 1.0 to 1.7 and 3.5 to 5.5 mu g/kg, respectively. Finally, the proposed method was applied to analyze beeswax samples collected from experimental and organic apiaries.
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