Journal
JOURNAL OF SEPARATION SCIENCE
Volume 38, Issue 2, Pages 187-196Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201401131
Keywords
Fluorescent labeling; Fruits; High-performance liquid chromatography; Plant hormones
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Funding
- National Natural Science Foundation of China [31301595, 21475074, 21275089]
- Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety [GDPKLAPPS1401]
- Natural Science Foundation of Shandong Province, China [ZR2013BQ019]
- Qufu Normal University [bsqd 2012017]
- Soft Science Project of Shandong Province [2013RKA08020]
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Plant hormone determination in food matrices has attracted more and more attention because of their potential risks to human health. However, analytical methods for the analysis of multiple plant hormones remain poorly investigated. In the present study, a convenient, selective, and ultrasensitive high-performance liquid chromatography method for the simultaneous determination of multiple classes of plant hormones has been developed successfully using dispersive liquid-liquid microextraction followed by precolumn fluorescent labeling. Eight plant hormones in fruits including jasmonic acid, 12-oxo-phytodienoic acid, indole-3-acetic acid, 3-indolybutyric acid, 3-indolepropionic acid, gibberellin A(3), 1-naphthylacetic acid, and 2-naphthaleneacetic acid were analyzed by this method. The conditions employed for dispersive liquid-liquid microextraction were optimized systematically. The linearity for all plant hormones was found to be >0.9993 (R-2 values). This method offered low detection limits of 0.19-0.44 ng/mL (at a signal-to-noise ratio of 3), and method accuracies were in the range of 92.32-103.10%. The proposed method was applied to determine plant hormones in five kinds of food samples, and this method can achieve a short analysis time, low threshold levels of detection, and a high specificity for the analysis of targeted plant hormones present at trace level concentrations in complex matrices.
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