Journal
JOURNAL OF SEPARATION SCIENCE
Volume 37, Issue 4, Pages 404-411Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201301102
Keywords
Chemometrics; Curcumae longae rhizome; Essential oil; GC-MS; Quality control
Categories
Funding
- National Science Foundation of China [81274072]
- PUMC Youth Fund
- Fundamental Research Funds for the Central Universities [3332013078]
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Curcumae longae rhizome is a widely used traditional herb in many countries. Various geographical origins of this herb might lead to diversity or instability of the herbal quality. The objective of this work was to establish the chemical fingerprints for quality control and find the chemical markers for discriminating these herbs from different origins. First, chemical fingerprints of essential oil of 24 C. longae rhizome from four different geographical origins in China were determined by GC-MS. Then, pattern recognition techniques were introduced to analyze these abundant chemical data in depth; hierarchical cluster analysis was used to sort samples into groups by measuring their similarities, and principal component analysis and partial least-squares discriminate analysis were applied to find the main chemical markers for discriminating these samples. Curcumae longae rhizome from Guangxi province had the highest essential oil yield (4.32 +/- 1.45%). A total of 46 volatile compounds were identified in total. Consistent results were obtained to show that C. longae rhizome samples could be successfully grouped according to their origins, and turmerone, ar-turmerone, and zingiberene were the characteristic components for discriminating these samples of various geographical origins and for quality control. This finding revealed that fingerprinting analysis based on GC-MS coupled with chemometric techniques could provide a reliable platform to discriminate herbs from different origins, which is a benefit for quality control.
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