Journal
JOURNAL OF SEPARATION SCIENCE
Volume 36, Issue 16, Pages 2611-2614Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201300407
Keywords
High-performance counter-current chromatography; High-speed counter-current chromatography; Pimpinella anisum; Terpenoids
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High-performance counter-current chromatography was successfully used for the isolation and purification of terpenoid compounds from the essential oil of Pimpinella anisumL. A two-phase solvent system composed of n-heptane/methanol/ethyl acetate/water (5:2:5:2, v/v/v/v) was suitable for the purification of linalool, terpinen-4-ol, -terpineol, p-anisaldehyde, while n-heptane/methanol (1:1, v/v) was used for the isolation of anethole and foeniculin. A scale-up process from analytical to preparative was developed. Additionally, a stepwise gradient elution was applied and instead of two different runs, 40 min each, one 80 min separation was performed; although the time of separation remains the same, it was possible to repeat the efficiency even if the water-containing mobile phase was changed to a nonaqueous system. The obtained essential oil, as well as purified compounds, was analyzed by GC. A total of 0.64 mg of linalool, 0.52 mg of terpinen-4-ol, 0.10 mg of -terpineol, 0.62 mg of p-anisaldehyde, 15 mg of anethole, and 2.12 mg of foeniculin were obtained from 210 mg of the essential oil of P. anisumL. in a short time with purities of 99, 98, 94, 93.54, 93, and 93.6%, respectively.
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