Journal
JOURNAL OF SEPARATION SCIENCE
Volume 36, Issue 12, Pages 1953-1958Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201300012
Keywords
Aconitum alkaloids; Baifupian; Fuzi; Heishunpian; Rapid resolution LC
Categories
Funding
- National Natural Science Foundation of China [81001597]
- China Academy of Chinese Medicine Sciences [zz2012011]
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A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra- and interday precisions (RSD < 5.07%) with good accuracies (97.76-105.08%) and good recovery (95.0-107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30-17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.
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